HIC Chromatography Media UniHR Phenyl-30S

   Nanomicro Tech is devoted to developing world-leading technologies for the production of the highest quality and the widest selection of uniformly sized nano- and microspheres (microparticles) .The unique feature of HIC is that proteins bind at high salt concentration and elute at low salt concentration. While in reversed phase column chromatography (RPC), desorption is often promoted by the addition of an increasing amount of organic solvent. Therefore gentle binding and elution condition of HIC allows a better retaining of the biologic activity of the target protein than RPC in which organic solvents are applied.      The strength of the hydrophobic interaction is influenced strongly by the nature of the salt components in the mobile phase. The salt concentration needed depends on the protein hydrophobicity and solubility. HIC is particularly attractive for protein purification after ion exchange and affinity chromatography, where th…