HIC Chromatography Media Uni-HR Phenyl-80S

  Nanomicro Tech offers a series of hydrophobic interaction chromatography (HIC) media for high efficiency separation and purification of proteins and peptides. HIC is used to separate proteins on the basis of relative hydrophobicity.Therefore gentle binding and elution condition of HIC allows a better retaining of the biologic activity of the target protein than RPC in which organic solvents are applied.     The strength of the hydrophobic interaction is influenced strongly by the nature of the salt components in the mobile phase. The salt concentration needed depends on the protein hydrophobicity and solubility. HIC is particularly attractive for protein purification after ion exchange and affinity chromatography, where the sample with high salt concentration can often be applied directly to HIC. By combining HIC with ion exchange or affinity chromatography, the purification of biomolecules can be completed efficiently.  …